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olfm4 intestinal stem cell  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc olfm4 intestinal stem cell
    Olfm4 Intestinal Stem Cell, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/olfm4 intestinal stem cell/product/STEMCELL Technologies Inc
    Average 90 stars, based on 1 article reviews
    olfm4 intestinal stem cell - by Bioz Stars, 2026-03
    90/100 stars

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    Antibodies used for immunofluorescence of the ileum.

    Journal: PLoS ONE

    Article Title: Treatment of necrotizing enterocolitis by conditioned medium derived from human amniotic fluid stem cells

    doi: 10.1371/journal.pone.0260522

    Figure Lengend Snippet: Antibodies used for immunofluorescence of the ileum.

    Article Snippet: Primary OLFM4 , Intestinal stem cells , Rabbit , 1:500 , Cell Signalling Technology (Danvers, MS, USA) , 39141.

    Techniques: Immunofluorescence, Fluorescence

    (A) Survival curves for mice pups from the 3 groups on postnatal days 5 to 9. (B) Representative histological images of the terminal ileum. (C) Histological score of intestinal injury. Inflammatory gene marker expression of (D) Il-6 and (E) Tnf-α . Immunofluorescent protein expression of (F) MPO and (G) CC3 in the terminal ilea of the 3 groups. Yellow arrows indicate positive staining. Relative quantification of the (H) MPO and (I) CC3 protein expressions. (J) Angiogenesis/vascularization Vegf gene expression in the 3 groups. Data are presented as mean ± standard error, with significance of group comparisons based on one-way ANOVA and Tukey post-hoc tests. n = 10 for each group, *p<0.05, **p<0.01, and ***p<0.001.

    Journal: PLoS ONE

    Article Title: Treatment of necrotizing enterocolitis by conditioned medium derived from human amniotic fluid stem cells

    doi: 10.1371/journal.pone.0260522

    Figure Lengend Snippet: (A) Survival curves for mice pups from the 3 groups on postnatal days 5 to 9. (B) Representative histological images of the terminal ileum. (C) Histological score of intestinal injury. Inflammatory gene marker expression of (D) Il-6 and (E) Tnf-α . Immunofluorescent protein expression of (F) MPO and (G) CC3 in the terminal ilea of the 3 groups. Yellow arrows indicate positive staining. Relative quantification of the (H) MPO and (I) CC3 protein expressions. (J) Angiogenesis/vascularization Vegf gene expression in the 3 groups. Data are presented as mean ± standard error, with significance of group comparisons based on one-way ANOVA and Tukey post-hoc tests. n = 10 for each group, *p<0.05, **p<0.01, and ***p<0.001.

    Article Snippet: Primary OLFM4 , Intestinal stem cells , Rabbit , 1:500 , Cell Signalling Technology (Danvers, MS, USA) , 39141.

    Techniques: Marker, Expressing, Staining, Quantitative Proteomics, Gene Expression

    NEC induced an intestinal damage, increased inflammation (IL-6 and TNF-α), neutrophil infiltration (MPO), and elevated apoptosis (CC3), as well as decreased angiogenesis or vascularization (VEGF). Further, it diminished epithelial proliferation (Ki67) and stem cell regeneration activity (Lgr5 and Olfm4), however, CM administration reverses all of these effects to yield reduced intestinal injury and improved survival.

    Journal: PLoS ONE

    Article Title: Treatment of necrotizing enterocolitis by conditioned medium derived from human amniotic fluid stem cells

    doi: 10.1371/journal.pone.0260522

    Figure Lengend Snippet: NEC induced an intestinal damage, increased inflammation (IL-6 and TNF-α), neutrophil infiltration (MPO), and elevated apoptosis (CC3), as well as decreased angiogenesis or vascularization (VEGF). Further, it diminished epithelial proliferation (Ki67) and stem cell regeneration activity (Lgr5 and Olfm4), however, CM administration reverses all of these effects to yield reduced intestinal injury and improved survival.

    Article Snippet: Primary OLFM4 , Intestinal stem cells , Rabbit , 1:500 , Cell Signalling Technology (Danvers, MS, USA) , 39141.

    Techniques: Activity Assay